Coronavirus novel kalebu genus B.COVID-19 minangka penyakit infèksi pernapasan akut.Wong umume rentan.Saiki, pasien sing kena infeksi koronavirus minangka sumber utama infeksi;wong sing kena infeksi asimtomatik uga bisa dadi sumber infeksi.Adhedhasar penyelidikan epidemiologis saiki, periode inkubasi yaiku 1 nganti 14 dina, biasane 3 nganti 7 dina.Manifestasi utama kalebu demam, lemes lan batuk garing.Nasal congestion, irung runny, perih tenggorokan, myalgia lan diare ditemokake ing sawetara kasus.
Kit iki digunakake kanggo deteksi kualitatif in vitro novel coronavirus 2019-nCov ing spesimen pernapasan kalebu swab oropharyngeal, sputum, cairan lavage bronchoalveolar lan swab nasofaring.Set primer lan probe label FAM dirancang kanggo deteksi spesifik gen ORFlab saka 2019-nCov.Probe berlabel VIC kanggo gen N saka 2019-nCov.Gen RNase P manungsa sing diekstrak bebarengan karo sampel tes menehi kontrol internal kanggo ngesyahke prosedur ekstraksi nukleik lan integritas reagen.Probe nargetake gen RNase P manungsa diwenehi label CY5.
| Komponen | 50 tes / kit |
| Reagen Campur Reaksi RT-PCR | 240μL × 1 tabung |
| Reagen Campur Enzim | 72μL × 1 tabung |
| Probe primer 2019-nCoV | 48μL × 1 tabung |
| Kontrol Positif | 200μL × 1 tabung |
| Kontrol Negatif | 200μL × 1 tabung |
Sensitivitas: 200 eksemplar / mL.
Spesifisitas: Ora ana reaksi silang karo SARS-CoV, MERS-CoV, CoV-HKU1, CoV-OC43, CoV-229E, CoV-NL63 lan HIN1, H3N2, H5N1, H7N9, Influenza B, Virus Parainfluenza (123), Rhinovirus(A). ,B,C), Adenovirus (1,2,3,4,5,7,55), Human interstitial pneumovirus, Human metapneumovirus, EBv, Measles virus, Human cytomegalic virus, Rota virus, Norovirus, Mumps virus, Varicella Zoster Virus , Mycoplasma pneumonia, Chlamydia pneumonia, Legionella, Bordetella pertussis, Haemophilus influenza, Staplhylococcus Aureus, Streptococcus Pneumonia, Streptococcus pyogenes, Klebsiella pneumonia, Tuberculous bacillus, Aspergillus fumigatus, Candida albicans, Cryptococcus glabrataform.
Presisi: CV ≤5%.
Sistem PCR Real-Time: Aehealth Diagenex AL, ABI 7500, ViiATM7, Quant Studio 7 fleksibel. Roche Lightcycler 480, Agilent Mx3000P/3005P, Rotor-GeneTM6000/0.Bio-Rad CEX96 TutulTMSLAN-96S.SLAN-96P